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tumor-derived exosomes sas  (SAS institute)


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    Structured Review

    SAS institute tumor-derived exosomes sas
    Tumor Derived Exosomes Sas, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sas-derived+exosomes/sas+derived+exosomes/pm40266314-1868-18-28
    Average 90 stars, based on 1 article reviews
    tumor-derived exosomes sas - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Membrane:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Labeling:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Protease Inhibitor:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Imaging:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Injection:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Expressing:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Concentration Assay:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Flow Cytometry:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Quantitative RT-PCR:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Control:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Derivative Assay:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Transmission Assay:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Electron Microscopy:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Software:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Saline:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Transmission Electron Microscopy:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).

    Negative Control:

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was be- Separations 2022, 9, 435 6 of 11 tween the value of the positive and negative control).

    Article Title: Optimization Protocol of the PEG-Based Method for OSCC-Derived Exosome Isolation and Downstream Applications
    Article Snippet: We successfully detected U6 expression in SAS- and OECM-1-derived exosomes (a single-peak melting curve and Ct value was between the value of the positive and negative control).

    Article Title: Epithelium-derived exosomal dipeptidyl peptidase-4 involved in arecoline-induced oral submucous fibrosis.
    Article Snippet: Mg63 cells and primary mouse oral fibroblasts were treated with 20 μg of SAS-derived exosomes (pre-treated with 0, 50, or 100 μg/mL arecoline) with or without the addition of 1 mM diprotin A or 100 μM sitagliptin.

    Article Title: Hypoxia-induced long non-coding RNA HIF1A‑AS2 regulates stability of MHC class I protein in head and neck cancer
    Article Snippet: 1 Hypoxia-induced long non-coding RNA HIF1A‐AS2 regulates stability of MHC class I protein in head and neck cancer Tsai-Tsen Liao 1,2,3,4* , Yu-Hsien Chen 5 , Zih-Yu Li 5 , An-Ching Hsiao 1 , Ya-Li Huang 5 , Ruo-Xin Hao 5 , Shyh-Kuan Tai 6 , Pen-Yuan Chu 6 , Jing-Wen Shih 2,7,8 , Hsing-Jien Kung 2,7,8,9,10 , and Muh-Hwa Yang 5,11,12,13* 1 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan 2 Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan 3 Cell Physiology and Molecular Image Research Center, Wan Fang Hospital, Taipei Medical University, New Taipei City 235, Taiwan 4 Cancer Research Center, Taipei Medical University Hospital, Taipei, Taiwan 5 Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan 6 Department of Otolaryngology, Taipei Veterans General Hospital, Taipei 112, Taiwan 7 Ph.D.. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan D ow nloaded from http://aacrjournals.org/cancerim m unolres/article-pdf/doi/10.1158/2326-6066.C IR -23-0622/3469618/cir-23-0622.pdf by guest on 06 July 2024 2 8 Ph.D.. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

    Article Title: Hypoxia-Induced Long Noncoding RNA HIF1A- AS2 Regulates Stability of MHC Class I Protein in Head and Neck Cancer
    Article Snippet: Flow cytometry results demonstrated the engulfment of SAS-derived exosomes (SAS-TEXs) into SG cells (Supplementary Fig. S9B).



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